This is topic Radiolabeled Antibodies: in forum Books, Films, Food and Culture at Hatrack River Forum.


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Posted by Phanto (Member # 5897) on :
 
Quick question. Can radiolabeled antibodies be used in tranmission electron microscopy (TEM)? I can't find definitive evidence in pubmed for or against. A reference would be great.

Thanks!
 

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Posted by ClaudiaTherese (Member # 923) on :
 
Do you need human research or will mice suffice?
 

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Posted by ketchupqueen (Member # 6877) on :
 
Do your own homework!



I have no clue but had to say it.
 

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Posted by ClaudiaTherese (Member # 923) on :
 
If you just need an incidental citation, this may do: Effects of 131I-labeled TNT-1 radioimmunotherapy on HT-29 human colon adenocarcinoma spheroids. It is human research published in 1991 via Cancer Immunology, Immunotherapy.

quote:

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Radiolabeled murine monoclonal antibody TNT-1, directed against the nuclear histones of degenerating cells, was used to treat human colon adenocarcinoma HT-29 spheroids in vitro. The therapeutic effects of 131I-TNT-1 were investigated as a function of the radioactive dose, treatment time, and number of treatments. Efficacy of treatment was assessed by TNT-1 antibody uptake, spheroid growth delay, and morphological examination using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM).... At the ultrastructural level, destruction of cells in the treated spheroids included the formation of porous cell membranes, crater-like holes (SEM), blebbing, and dissolution of cytoplasmic organelles (TEM).

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quote:

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Originally posted by ketchupqueen:
Do your own homework!



I have no clue but had to say it.

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And yeah, what she said. *grin

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PS: When you say "in transmission electron microscopy," I don't know if you mean "by" or something like "in conjunction with." The correct answer may differ with respect to such interpretation, and I leave that distinction (if any) as an exercise for the reader.
 

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Posted by Phanto (Member # 5897) on :
 
Meh, I'm trying to argue that radiolabeled antibodies could be used with TEM to visualize proteins on a cell surface, while that reference talks about observing efficacy of treatment.

I don't think I'm going to win this argument tho. Thanks anyway!
 

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Posted by ClaudiaTherese (Member # 923) on :
 
De nada. Best of luck, regardless.

I don't know whether this will be of help. I haven't read through to seen when radiolabelling is used and when it is fluorescence.

quote:

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Here, we consider the influence of selected tumor parameters on radioimmunotherapy, by comparing the gross biodistribution, microdistribution, and therapeutic efficacy of either radiolabeled or fluorescently labeled antibodies (A5B7 anti–carcinoembryonic antigen antibody and a nonspecific control) after i.v. injection in two contrasting human colorectal xenografts in MF1 nude mice.... Three-dimensional microvascular corrosion casting and transmission electron microscopy showed further structural differences between xenografts.

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quote:

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Originally posted by Phanto:
Meh, I'm trying to argue that radiolabeled antibodies could be used with TEM to visualize proteins on a cell surface, while that reference talks about observing efficacy of treatment.

I don't think I'm going to win this argument tho. Thanks anyway!

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