posted
Quick question. Can radiolabeled antibodies be used in tranmission electron microscopy (TEM)? I can't find definitive evidence in pubmed for or against. A reference would be great.
quote:Radiolabeled murine monoclonal antibody TNT-1, directed against the nuclear histones of degenerating cells, was used to treat human colon adenocarcinoma HT-29 spheroids in vitro. The therapeutic effects of 131I-TNT-1 were investigated as a function of the radioactive dose, treatment time, and number of treatments. Efficacy of treatment was assessed by TNT-1 antibody uptake, spheroid growth delay, and morphological examination using light microscopy, scanning electron microscopy (SEM), and transmission electron microscopy (TEM).... At the ultrastructural level, destruction of cells in the treated spheroids included the formation of porous cell membranes, crater-like holes (SEM), blebbing, and dissolution of cytoplasmic organelles (TEM).
A detailed discussion of method would probably require an immunology or radiotherapy textbook.
Posts: 14017 | Registered: May 2000
| IP: Logged |
quote:Originally posted by ketchupqueen: Do your own homework!
I have no clue but had to say it.
And yeah, what she said. *grin
---
PS: When you say "in transmission electron microscopy," I don't know if you mean "by" or something like "in conjunction with." The correct answer may differ with respect to such interpretation, and I leave that distinction (if any) as an exercise for the reader. Posts: 14017 | Registered: May 2000
| IP: Logged |
posted
Meh, I'm trying to argue that radiolabeled antibodies could be used with TEM to visualize proteins on a cell surface, while that reference talks about observing efficacy of treatment.
I don't think I'm going to win this argument tho. Thanks anyway!
Posts: 3060 | Registered: Nov 2003
| IP: Logged |
I don't know whether this will be of help. I haven't read through to seen when radiolabelling is used and when it is fluorescence.
quote:Here, we consider the influence of selected tumor parameters on radioimmunotherapy, by comparing the gross biodistribution, microdistribution, and therapeutic efficacy of either radiolabeled or fluorescently labeled antibodies (A5B7 anti–carcinoembryonic antigen antibody and a nonspecific control) after i.v. injection in two contrasting human colorectal xenografts in MF1 nude mice.... Three-dimensional microvascular corrosion casting and transmission electron microscopy showed further structural differences between xenografts.
quote:Originally posted by Phanto: Meh, I'm trying to argue that radiolabeled antibodies could be used with TEM to visualize proteins on a cell surface, while that reference talks about observing efficacy of treatment.
I don't think I'm going to win this argument tho. Thanks anyway!
Why do you want to use radio labeled antibodies? Most of elements used for radio labeling proteins aren't going to show up any better in a TEM images than the original protein. A colloidal gold label would be far more effective than a radio label.
Posts: 12591 | Registered: Jan 2000
| IP: Logged |
Printer-friendly view of this topic
![[Smile]](smile.gif)
![[Wink]](wink.gif)
![[Big Grin]](biggrin.gif)
